Resources
Activities
Mastering High Content Screening: From Theory to Practice
The "Mastering High-Content Screening: From Theory to Practice" workshop is a precious event that trends us to enable precision medicine for cancer patients through high-content screening techniques.
Day 1: Highlights with an overview of HCS technologies by Revvity. It introduces us to how to apply high-throughput technologies to pre-clinical animal imaging in the therapeutics discovery pipeline.
Day 2: Hands-on sessions for direct interaction with Harmony software for image analysis and PreciScan technology for rapid object recognition.
Lecture Series on Animal Research “Animal research for skin diseases”
On February 7th, 2024, our laboratory had the esteemed privilege of hosting Professor Srikala Raghavan from A*STAR Skin Research Labs (A*SRL), Institute for Stem Cell Science and Regenerative Medicine, Singapore and India.
With a wealth of knowledge and experience under her belt, Professor Raghavan graced us with a captivating lecture series titled "Animal Research for Skin Diseases." Her visit not only illuminated the intricacies of dermatological research but also left an indelible mark on our understanding of the vital role that animals play in advancing our comprehension and treatment of skin disorders.
Tools
CCAexplorer
This repository contains the code for our cholangiocarcinoma project, titled 'Comprehensive Drug Response Profiling and Pan-Omic Analysis: Identifying Therapeutic Candidates and Prognostic Biomarkers for Asian Cholangiocarcinoma'.
MG Calculator
Wound healing assay is a simple and cost-effective in vitro assay for assessing therapeutic impacts on cell migration. Its major limitation is the possible confoundment by other cellular phenotypes, causing misinterpretation of the experimental outcome. Here, we developed a novel methodology that enables the quantification of therapeutic impacts on different cellular phenotypes. Our technique requires only the concurrent measurement of cell death together with the relative wound density changes and is easily adapted for high-throughput drug screening. By using context-dependent wound closure time, our approach can minimize influences of extrinsic noises such as cell seeding density, ensuring that more robust inference of therapeutic effect on different phenotypic outcomes.
Multiplex Imaging Analysis (MCMICRO)
MCMICRO is an end-to-end processing pipeline for multiplexed whole slide imaging and tissue microarrays developed at the HMS Laboratory of Systems Pharmacology. It comprises stitching and registration, segmentation, and single-cell feature extraction. Each step of the pipeline is containerized to enable portable deployment across an array of compute environments.
We installed the MCMICRO, created the pipeline, and ran on local system adding additional tools such as Scimap